Kholoud Samir Mohamed 152503 <br/><br/>
Validated Chromatographic Methods for Determination of  Drugs in Edible Animal Tissues // GP //DR. Manal Fouad  (2018 - 2019 ) 

 - Giza   MSA  2019 
 - 50 
 - Pharmacy  DISTINGUISHED PROJECTS 2019 .
<br/><br/> Pharmacy 
<br/><br/> Sensitive methods are considered to be essential in the analysis of drug residues in<br/>complex animal tissue matrices. The main hazard of such residues is the development<br/>of antimicrobial resistance that may be transferred to the consumers. Accordingly, the<br/>target of our project is mainly the development of validated, sensitive and simple<br/>chromatographic methods aiming for the determination of trimethoprim (TMP) and<br/>sulfadiazine (SDZ) in their pure forms and in residues of meat tissue. Two methods<br/>were developed and validated, through which a comparative study was performed.<br/>The first method is a RP- liquid chromatographic method while the second one is a<br/>miceller chromatographic method. Validation was performed according to the<br/>International Conference of Harmonization guidelines (ICH).Linearity of the studied<br/>drugs covered the range of 0.5-60 μg/mL for SDZ and 0.5-70 μg/mL for TMP by the<br/>first method and in the range of 0.5-100 μg/mL for both drugs using the second<br/>method. The developed methods showed good recovery, repeatability, and sensitivity.<br/>Moreover, high extraction efficiency was obtained without matrix interference in the<br/>extraction process and in the subsequent chromatographic determination. Statistical<br/>comparison of the obtained results compared with those of a reported method revealed<br/>good agreement and proved that there was no significant difference in the accuracy<br/>and precision between the reported and the two developed methods. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Chromatographic Methods<br/>Determination of  Drugs
<br/><br/><label>Dewey Class. No.: </label>615.19