000			02886nam a22002297a 4500
999			_c13914 _d13914
003			OSt
005			20190306175324.0
008			180719b xxu||||| |||| 00| 0 eng d
040			_cMSA
082			_a615.31 _bA.A.M 2018
100			_aAlaa Attia Abdelrauof Attia. 152261
245			_aMonitoring the anti-cancer potential of gold nanoparticles under the influence of electroporation and ultra-low electromagnetic field (ULEMF) frequencies relative to time on colon cancer cell-lines (HCT-116) مراقبة القدرة المضادة للسرطان للجزئيات النانوية الذهبية تحت تأثير التثقيب الكهربائي والترددات الكهرومغناطيسية المنخفضة )ULEMF )نسبيا للوقت على خاليا سرطان القولون )HCT-116( \\GP\\DR. Riham Mohsen ( SPRING 2018 ) _cAlaa Attia Abdelrauof Attia.
260			_aGiaz : _bMSA, _c2018.
300			_a79 p. :, _b26 CM.
440			_aBiotechnology DISTINGUISHED PROJECTS 2018
520			_aThe use of nanotechnology in cancer therapy is based on the usage of molecules that can affect cancerous cells as well as mitochondrial membrane in order to induce cancer cell programmed death. This method has shown overwhelming effects; hence the target of this project is to evaluate the role of gold nanoparticles’ anti-cancer potential under the effect of electroporation, in addition to the effect of ultra-low electromagnetic field (ULEMF) frequencies on HCT 116 colon cancer cell line. It was noticed that this method of using ULEMF displayed a higher potential of anti-cancer activity than the use of gold nanoparticles used single handed (engulfed endocytosis). At the same time cell apoptosis that is accompanied with high percentage of cell arrest in case of treatment with electroporation, gold nanoparticles as well as electromagnetic field of 50 mT for 30 mins showed the best results in comparison with other treatments. Also, it has been signified that the usage of these treatment combinations resulted in a highly elevated gene expression of pro-apoptotic genes (P53, Bax) and down regulation of anti-apoptotic gene (Bcl-2). It was also noticed that an arrest of cells occurred at the G2/M phase compared to other treatment regimes. Data recorded revealed that the use of gold nanoparticles, electroporation and electromagnetic field in a dose dependent manner can increase apoptosis and necrosis [200v]- [25 mT— 50 mT]. It can be suggested that the use of electromagnetic field as well as, gold nanoparticles and electroporation can be a promising tool for cancer
650			_aanti-cancer potential of gold .
650			_a(ULEMF).
700			_aAly Fahmy. _eExternal Supervisor
856			_uhttps://drive.google.com/file/d/1FYIWXQdRaHA00t9pt2yjJse9Y-XJtROF/view?usp=sharing _zFULL TEXT PRESS HERE
942			_2ddc _cGP