000			02714nam a22002417a 4500
999			_c13923 _d13923
003			OSt
005			20190307085557.0
008			180719b xxu||||| |||| 00| 0 eng d
040			_cMSA
082			_a615.71 _bA.A.I 2018
100			_aAya Ali Mohamed. 150423
245			_aThe investigation of antiviral mechanisms of chitosan nanoparticles encapsulating curcumin against Hepatitis C Virus genotype – 4a on human hepatoma cell lines اآلليات المضادة للفيروسات من جسيمات ال Chitosan النانوية المغلفة بالكركمين ضد النمط الجيني لفيروس االلتهاب الكبدي سي - 4 أ في خالياالكبدالبشرية \\GP\\Dr. Hossam Taha. ( SPRING 2018 ) _cAya Ali Mohamed.
260			_aGiza: _bMSA University, _c2018.
300			_a130 P. :, _b26 CM.
440			_aBIOTECHNOLOGY DISTINGUISHED PROJECTS 2018
520			_aCurrent double and triple treatments with direct acting antivirals (DAAs) were recently reported to cause several side effects and thereby effective, less toxic antiviral agents are still needed. Accordingly, this study aimed at investigating the role of some natural and synthetic antivirals; curcumin, chitosan nanoparticles (CSNPs) and chitosan encapsulating curcumin nanocomposite on HCV-G4 replication in Huh7.5 cells. Viral Replication was quantified in HCV/G4 infected Huh7.5 cells either treated or not with curcumin, chitosan nanoparticles (CSNPs) and chitosan encapsulating curcumin nanocomposite using quantitative real time PCR assay. Nanocomposite (chitosan nanoparticles encapsulating curcumin) was successfully prepared and controlled to the size of 15 nm and charges of 25 mV, characterized by TEM, XRD, FTIR. Curcumin was encapsulated at concentration of 42mg/gm of chitosan nanoparticles. Chitosan nanoparticles at concentration of 100μg/mL showed almost 100% reduction of viral replication whereas the non-toxic dose of curcumin (13 μm) showed 50% reduction in the viral initial titer as detected by real-time PCR assay. Combining both agents in the nanocomposite led to a higher reduction in viral replication. Nanocomposite showed a significant reduction in viral replication when compared to curcumin and chitosan alone which suggests the potential role of nanocomposite as a novel, safe and effective antiviral agent. Keywords: curcumin, chitosan, nanocomposite, HCV – 4.
630			_vMedical Biotechnology
650			_aantiviral mechanisms.
650			_aHepatitis C.
700			_aSamah Loutfy. _eExternal Supervisor
856			_uhttps://drive.google.com/file/d/1ferD8oFQTYASOVFbG2JNrYKbA-XeCj1G/view?usp=sharing _zFULL TEXT PRESS HERE
942			_2ddc _cGP