000			02162nam a22002177a 4500
999			_c13929 _d13929
003			OSt
005			20190306180352.0
008			180721b xxu||||| |||| 00| 0 eng d
040			_cMSA
082			_a610.28
100			_aEmad Adel Bassily ( 152721 )
245			_aCytogenetics study in cases of gender crisis دراسه للعلم الوراثى للخلايا فى ازمات نوع الجنس // GP // DR / Ahmed Aref // Dr. Ola Khalifa // Dr. Osama Zaki( SPRING 2018 ) _b152721
260			_aGIZA _bMSA _c2018
300			_a89 P _b25 CM
440			_aBIOTECHNOLOGY DISTINGUISHED PROJECTS 2018
520			_aDisorder of sex development (DSD) are congenital conditions characterized by atypical development of chromosomal, gonadal, and phenotypic sex, DSD is a rare disease that may affect both male and female with a frequency of 1 in 20,000-25,000 in males, while the female prevalence is 1 in 16,000, as female with disorder in sex development may have uterus but with secondary sexual characters resemble those of males and this is due to the increase levels of the androgen hormone during pregnancy and the karyotyping will be 46 chromosomes that includes 44 autosomal chromosome and XY sex chromosomes instead of XX sex chromosomes of a normal female. This project aims to karyotype three patients one male and two females that was expected to have disorder of sex development, through six steps which starts by set up Harvest that starts by set up step to enable cell growth for 72 hrs. Then followed by harvest of the white blood cells, then Spreading, after that Banding step, Photo, and Karyotyping to detect if there is any gender crisis within this sample or not, for example if there is a female with (XY) instead of (XX) and male with (XX) instead of (XY) in their sex Chromosomes. While the results were positive for the three patients as the two females karyotype was 46, XY and the male karyotype
610			_vCytogenetics _vgender crisis
630			_vMedical Biotechnology
856			_uhttps://drive.google.com/file/d/1nepQjeE1bpwNmm3YwzfzQqzRlAYGwS6B/view?usp=sharing _zFULL TEXT PRESS HERE
942			_2ddc _cGP