000			02448nam a22002537a 4500
999			_c24969 _d24969
003			OSt
005			20190728115021.0
008			180715b xxu||||| |||| 00| 0 eng d
040			_cMSA
082			_a616.1
100			_aAmr Mohamed Alaa 150557
245			_aTHE EFFECT OF MODULATION OF RNA EXPRESSION ON MYOCARDIAL INFARCTION RSPHO8.2 // GP // Dr. Mai Amin Zaafan (2018 - 2019)
260			_aGiza : _bMSA, _c2019.
300			_a29 p.
440			_aPHARMACY DISTINGUISHED PROJECTS 2019
500			_aPharmacy - Pharmacology
520			_aMyocardial infarction (MI) is myocardial cell death due to severe and prolonged ischemia produced from atherosclerosis-related coronary artery disease. MI triggers a cascade of events and reparative phases end with myocardial cell necrosis. MicroRNA (miR) is non-coding single stranded RNA that regulates protein expression. miR-103 is used to regulate expression of Fas- associated death domain (FADD) which decreases necroptosis of ischemic myocardium. The study aims to investigate the modulatory effect of regulating mRNAs translation processes of myocardial infarction induced with Isoprenaline HCL 100 mg/kg (ISO) by injecting miR-103 inhibitor. Eighteen mice (15-25 gm) were allocated into three groups; Group A (control) received normal saline, Group B received ISO and Group C received ISO and miR-103 inhibitor. Mice were scarified by cervical dislocation under urethane anesthesia. Blood and hearts samples were collected for biochemical analysis of miR103, FADD, RIPK, IL-6 and Troponin-I. In addition, hearts were used for histopathological examination. Results showed that administration of miR-103 antagomir leads to increase in FADD protein levels in group C compared to group B. While miR-103, RIPK, and IL-6 showed high levels of expression in group B that is attenuated in group C. cardiac troponin-I also supported the previous results. Histopathological test showed normal histological structure in groups A and C while focal degeneration in myocardium in B. Accordingly, these results indicate a promising suppression of MI manifestations upon inhibition of miR-103.
650			_aMyocardial Infarction
700			_aAyman Selim Ahmed 151497
700			_aBana Ammar AlBarazi 154285
700			_aShahd Yehia AlWais 151775
856			_uhttps://drive.google.com/file/d/1qGSlsIjxJXrGonraH1WNNweQrg7pMvOo/view _zFULL TEXT PRESS HERE
942			_2ddc _cD.GP